Efficient DNA Extraction for HPV Genotyping in Formalin-Fixed, Paraffin-Embedded Tissues
نویسندگان
چکیده
منابع مشابه
Improvement of DNA Extraction for Human Papillomavirus Genotyping from Formalin-Fixed Paraffin-Embedded Tissues
Mucosal human papillomaviruses (HPVs) play a crucial role in the development of cervical carcinoma, the third most common malignancy in women worldwide. These viruses are classified as high risk (HR) or low risk (LR), depending on their transforming ability. Formalinfixed paraffin-embedded (FFPE) tissues represent the most frequent form of tissue storage in pathology departments. These archival...
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Formalin-fixed paraffin-embedded tissues (FFPET) represent the largest source of archival biological material available for genomic studies. In this work we present an advanced protocol for extraction of high quality DNA from FFPET that can be applied in several molecular studies. Although cat mammary tumours (CMT) are the third most frequent tumour in cats the recovery of significant number of...
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Formalin-fixed paraffin-embedded (FFPE) tissues are an important sample source for ret‐ rospective studies. Despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests since it fragments and chemically modifies nucleic acids, especially RNA. Although several studies describe techniques that allow extracting nu‐ cleic acids from FFPE tissues, so far the...
متن کاملComparison of Methods for the Extraction of DNA from Formalin-Fixed, Paraffin-Embedded Archival Tissues
AIM Discussing a protocol involving xylene-ethanol deparaffinization on slides followed by a kit-based extraction that allows for the extraction of high quality DNA from FFPE tissues. METHODS DNA was extracted from the FFPE tissues of 16 randomly selected blocks. Methods involving deparaffinization on slides or tubes, enzyme digestion overnight or for 72 hours and isolation using phenol chlor...
متن کاملDNA extraction from formalin-fixed, paraffin-embedded tissue.
Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for P...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2011
ISSN: 1525-1578
DOI: 10.1016/j.jmoldx.2011.03.007